Isolation of potential novel endospore-containing bacteria from Canada goose feces

Haley Keillor, Molly Svendsen

Abstract


Non-toxin producing anaerobic bacteria have been found to promote anti-inflammatory immune responses in the mammalian gut. Therefore, the isolation of chloroform resistant bacterial strains from Canada goose (Branta canadensis) feces could select for spore-forming bacteria with probiotic properties. Our working hypothesis is that the minimal result will be the isolation and identification of such bacterial species from Canada goose microflora. Three percent chloroform treatment of Canada goose feces was completed for one hour to select for chloroform resistant bacterial cells. Surviving cells were cultured in aerobic and anaerobic conditions to differentiate strains by metabolic strategy. Gram staining revealed twenty-six morphologically distinct endospore containing isolates. Twelve anaerobic Gram positive (3) and negative (9) isolates were obtained by culturing on Brucella blood with vitamin K and hemin or reinforced clostridial hiveg hydrolysate with L-cysteine, Na acetate and starch agars. Fourteen aerobic Gram positive (4) and negative (10) isolates were cultured using the two-aforementioned media and nine (9) aerobes could subsequently be propagated on lysogeny broth (LB). Results from these investigations further demonstrate that newly identified, potential probiotic bacterial cultures can be isolated from free-ranging species and identified. Current efforts are focusing on 16S ribosomal RNA sequencing, to determine the identification of our isolates and the potential presence of novel microbial species.

Keywords


antimicrobial; axenic bacterial culture; microbial ecology; free-ranging avian

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