Co-culture Screens for the Isolation of Antibiotics from Sarracenia Pitcher Plant Bacteria

Jackson Tweed

Abstract


New antibiotics must be continually developed to combat multidrug resistant bacteria. Bacterial co-culture has been previously described as a method to induce antibiotic production in microorganisms. Using a bacterial library of isolates from Sarracenia pitcher plants in Western North Carolina, a high throughput screening method was developed and optimized to identify bacteria that produce antibiotic compounds in either single culture or co-culture. For antibiotic producers, the culture was scaled up to liter volumes and the antibiotics were isolated using liquid-liquid extraction followed by chromatography. Work is ongoing to increase the yield of each compound, and to determine their structures using 1H NMR, 13C NMR, and IR spectroscopy.


Keywords


Co-culture; Antibiotics; bacteria

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