Development of a Mouse Model of Obesity via High Sucrose Consumption

Jared Wagoner, Emma Felzien, Calvin Vetter, Ken Johnson, Alec Rodriguez

Abstract


The purpose of this project was to generate a mouse model of obesity, glucose intolerance and insulin resistance using high sucrose consumption for undergraduate research of metabolism, obesity and diabetes. The goal was to achieve diet induced obesity in these animals within a single semester (approximately 4 months), so that organs could be harvested and studied in the following semester. Initially, fancy mice from a local breeder were placed on a 10% sucrose solution in place of water.  It was hypothesized that dietary intervention would lead to significant weight gain over the period of 12 weeks. However, 12 weeks was not sufficient to lead to a significant effect on bodyweight.  Therefore the study was repeated using a 30% sucrose solution and a commonly used mouse strain that is sensitive to diet induced obesity, the C57BL/6, was selected as the model.  These animals gained significantly more weight on the sucrose solution and were glucose intolerant by the end of 12 weeks as demonstrated by a glucose tolerance test.  However, an insulin tolerance test was performed and results from this test indicated that, while fasting blood glucose is elevated, insulin resistance was not confirmed at the end of 12 weeks.  While insulin sensitivity may need to be investigated in another manner, the results of this study indicate that a mouse model of obesity and glucose intolerance can be generated within a single semester, which makes this a useful model for undergraduate research of metabolism, obesity and potentially Type 2 Diabetes.


Keywords


Obesity; Glucose Intolerance; Insulin Resistance

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