Fluorescent Imaging of Rhizoctonia solani Isolate TOM7 Hyphae Anastomosis

Robert Paul Connacher


Samples of the filamentous fungi Rhizoctonia solani isolate strain TOM7 were stained with the nucleic acid-staining fluorescent dye acidrine orange in order to image hyphae anastomosis. Images were collected of TOM7 hyphae anastomosing with other TOM7 hyphae, which readily occured under stressful conditions (e.g., lack of nutrients, etc.). Furthermore, this "self-anastomosis" was not observed when nutrients were prevalent. Additionally, both incompatible and compatible pairs of R. solani hyphae were crossed in order to induce anastomosis. The results provided evidence that the hyphae never anastomosed with incompatible pairs (i.e., the hyphae remained parallel or crossed over without merging); and compatible pairs showed limited anastomosis. The images corroborate previous findings that hyphae may release diffusible factors such as viruses or toxins upon contact with hyphae of an incompatible strain.


Rhizoctonia solani; Anastomosis; Fluorescent Microscopy

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